The growing behavior of Candida albicans in various concentration of glucose and corticosteroid media was studied with the method of modified hanging slide culture.
The strains of Candida albicans used in this study were obtained from vaginal swab from outpatients and were isolated from cultured colonies on Sabouraud´s glucose agar media.
To detect the budding rate of blastospore, the diluted suspension of Candida albicans in normal saline were inoculated into various concentration of glucose (G1, G2, G3, G4), corticosteroid (Sl, S2, S3, S4) and corticosteroid with 10% pepton broth (Dl, D2, D3, D4) respectively and cultured at room temperature (22∼25℃).
The number of budding of blastospore were counted under the high power field of light microscope (400X) at specific time interval, e.g, 1, 2, 3, 6, 9, 12, 18, and 24 hours after inoculation.
The results are as following:
1. The most effective budding rate was seen in G4 media (1.25% glucose) in 18 hours aft inoculation (89%).
2. The budding rate in Sabouraud´s glucose broth with various concentration of dexamethasone added, was not significantly different from that of simple Sabouraud´s glucose broth within 18 hours after inoculation, but there was statistically significant difference in two budding rate at 24 hours observation.
3. The budding rate in 10% pepton broth media with various concentration of dexamethasone was almost same budding rate in control group, which is normal saline and 10% pepton broth, except on 2 and 24 hours results.
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